Allergy Alert Sign: Print and place on the outside of the doors to areas where animals are used. This sign is used to alert non-animal users to the potential for allergens and to provide information on how to get a respirator, if desired.
Emergency Contact Sign: Print, complete and place on the inside of the doors to areas where animals are used. This sign is used in the event that there is an emergency and first responders find animals in the lab. It also provides contact information for animal welfare issues, as required by regulation.
Animal Hazards Checklist: Print, complete and file as appropriate. This is part of the Occupational Health Program associated with animal use. The form is a prompt to look for hazards and to deal with them in a timely manner. On the yearly anniversary of your protocol, you will be asked to complete a Continuing Review. One of the questions will be a prompt to review the Animal Hazards Checklist.
Surgery and Post-Operative Monitoring Form: This is a sample surgical record. Feel free to use this form or adapt it to what works for you. These are the records that the IACUC review during the semi-annual inspections. Note that surgical records must be accessible during IACUC inspections. We suggest using a binder to keep all records centrally and making copies for individual lab notebooks.
Non-Surgical Analgesia Record: This is a samle non-surgical analgesia record. Same comments apply for this form as for the Surgery and Post-Operative Monitoring Form above.
Drugs: We look at storage, grade and expiration date of clinical drugs, e.g., isoflurane, ketamine, xylazine, ophthalmic ointment and surgical glue. These types of drugs should be pharmaceutical grade and not expired. A description of pharmaceutical grade for the IACUC purposes is found in IACUC Policy 210.
Ketamine and ketamine cocktails should be stored as for controlled substances (for more information contact RLSS). The IACUC do not inspect the DEA paperwork associated with controlled substances, but we are required that they are stored appropriately. Anesthetic cocktails must be stored and labelled as described in IACUC Policy 210.
Surgical location This should be away from heavy traffic areas, if at all possible. There should also be a physically separate area for preparation of the animal (hair removal, surgical scrub). This can be on the same bench, but it must be a distinct area from the surgical location.
CO2 euthanasia: IACUC Guidance 214 describes the type of chamber, CO2 source, flow meter and method for confirming death, that meet the regulations.
For an animal emergency, contact the on-call veterinarian. The phone numbers are posted in each University Animal Care animal facility. If the situation is not an emergency, contact one of the University Animal Care veterinarians.
Cumulative Index for Mammalian Species - summarizes the current understanding of the biology of a single species, including systematics, distribution, fossil history, genetics, anatomy, physiology, behavior, ecology, and conservation
The following article describes the development of the "mouse grimace scale", which is a method for determining levels of pain by examining facial expressions. The article includes clear pictures and descriptions of the facial expressions:
Langford, Bailey, Chanda, et al., (2010). Coding of facial expressions of pain in the laboratory mouse. Nature Methods, 7:447-449.
The following article describes methods for determining the health status of mice, and provides guidance on establishing study endpoints based on health status:
Ullman-Culleré and Foltz (1999). Body condition scoring: a rapid and accurate method for assessing health status in mice. Laboratory Animal Science, 49:319-323.
The articles can be accessed through E-Journals at the UA library.